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This article in CS

  1. Vol. 33 No. 6, p. 1131-1135
     
    Received: Dec 11, 1992


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doi:10.2135/cropsci1993.0011183X003300060003x

Cause of Tall Off-Types in a Semidwarf Spring Wheat

  1. E. W. Storlie and
  2. L. E. Talbert 
  1. Dep. of Plant and Soil Sci., Montana State Univ., Bozeman, MT 59717

Abstract

Abstract

Tall off-types that occur in semidwarf wheat, Triticum aestivum L., cultivars may reduce grower acceptance and present problems in seed certification programs. Tall off-types occur in ‘Hi-Line’ spring wheat at a frequency of 0.20%. Segregation analysis for height indicates Hi- Line contained the Rht1Rht1rhl2rht2 genotype. Cytological analysis of selfed progeny of the tall off-types showed a high proportion of monosomic chromosome numbers, indicating that aneuploidy may be associated with the tall off-types. Further investigation involved the use of PCR (polymerase chain reaction) with primers designed from clones detecting restriction fragment length polymorphism (RFLP) at loci on homoeologous Group 4, which contained height-reducing loci Rht, and Rht2. Three sets of primers were used to screen progeny of tall off-types from Hi-Line. The results of this screening indicate 20% of the offspring were nullisomic for chromosome 4B. Analysis of progeny using morphological and PCR based screening indicate that offspring of tall off-types were 72% monosomic 46:8% disomic: 20% nullisomic 4B. Thus, cytological and molecular analysis reveal that Hi-Line tall off-types result from a monosomic 4B condition, which causes hemizygosity for Rht1. Due to the random occurrence of monosomic 4B progeny from disomic semidwarf wheat, our results suggest that tall off-types may be an inherent and unavoidable feature of semidwarf wheats with the genotype Rht1,Rht1,rht2rht2.

Contribution no. J-2847 of the Montana Agric. Exp. Stn.

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