Plant Regeneration from St. Augustinegrass Immature Embryo-Derived Callus
- Yu-Jen Kuo and
- M. A. L. Smith
Development of efficient methods for plant regeneration from callus broadens the opportunities for genetic manipulation of new turfgrass selections through tissue culture. This study was conducted to induce callus formation and plant regeneration from embryo explants of St. Augustine grass [Stenotaphrum secundatum (Walt.) Kuntze ‘Texas Common’]. Immature embryo explants incubated in the dark produced yellow, amorphous callus on Murashige and Skoog (MS) agar solidified medium with 30 g L−1 sucrose and 1 or 5 mg L−1 dichlorophenoxyacetic acid (2,4-D). Subculture to medium with 0.25 L−1 2,4-D and 0.5 mg L−1 kinetin and introduction of a 14-h photoperiod under fluorescent lamps (16μmol−2 s−1 photosynthetic photon flux [PPF]) induced formation of white, friable, embryogenic callus. Scanning electronic microscopy revealed that plants regenerated via somatic embryogenesis. Complete plant regeneration was achieved from 33% of the original explants after transfer to 0.5 × MS medium without growth regulators. These rooted plantlets were transplanted to a soil mix in the glasshouse with 100% acclimation success. This system for efficient regeneration of St. Augustine grass from callus could be applied to improved cultivar selection after environmental stress testing at the cell level.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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