Extracting Volatile Compounds from Single Plants using Supercritical Fluid Extraction
- John A. Henning ,
- Robert J. Core and
- Jorge L. Gardea-Torresdey
Analysis of plant volatile compounds typically requires large amounts of plant tissue, thus preventing sampling from specific morphological regions of individual plants. We used supercritical fluid extraction (SFE), with CO2 as the solvent, to extract plant volatiles from 1.5 g of fresh alfalfa germplasm tissue (Medicago sativa L. and M. glandulosa David). More than 100 volatile and semi-volatile compounds were detected by gas chromatography-mass spectrometry (GS-MS); most of the these (>80) were in sufficient quantity for accurate spectral analysis. SFE is an efficient microextraction technique for volatile compounds contained within single plants.
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