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This article in CS

  1. Vol. 34 No. 4, p. 1120-1122
     
    Received: July 19, 1993


    * Corresponding author(s): jhenning@dante.nmsu.edu
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doi:10.2135/cropsci1994.0011183X003400040053x

Extracting Volatile Compounds from Single Plants using Supercritical Fluid Extraction

  1. John A. Henning ,
  2. Robert J. Core and
  3. Jorge L. Gardea-Torresdey
  1. Dep. of Agronomy and Horticulture, New Mexico State Univ., Las Cruces, NM 88003-0003

Abstract

Abstract

Analysis of plant volatile compounds typically requires large amounts of plant tissue, thus preventing sampling from specific morphological regions of individual plants. We used supercritical fluid extraction (SFE), with CO2 as the solvent, to extract plant volatiles from 1.5 g of fresh alfalfa germplasm tissue (Medicago sativa L. and M. glandulosa David). More than 100 volatile and semi-volatile compounds were detected by gas chromatography-mass spectrometry (GS-MS); most of the these (>80) were in sufficient quantity for accurate spectral analysis. SFE is an efficient microextraction technique for volatile compounds contained within single plants.

Research supported by the New Mexico State Agric. Exp. Stn. and Hewlett Packard Co. Univ. Grants.

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Copyright © 1994. Crop Science Society of America, Inc.Copyright © 1994 by the Crop Science Society of America, Inc.