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This article in CS

  1. Vol. 35 No. 5, p. 1451-1461
    Received: Jan 16, 1995

    * Corresponding author(s): srpadg@ccmail.monsanto.com


Development, Identification, and Characterization of a Glyphosate-Tolerant Soybean Line

  1. S. R. Padgette ,
  2. K. H. Kolacz,
  3. X. Delannay,
  4. D. B. Re,
  5. B. J. LaVallee,
  6. C. N. Tinius,
  7. W. K. Rhodes,
  8. Y. I. Otero,
  9. G. F. Barry,
  10. D. A. Eichholtz,
  11. V. M. Peschke,
  12. D. L. Nida,
  13. N. B. Taylor and
  14. G. M. Kishore
  1. Monsanto Co., 700 Chesterfield Parkway N., St. Louis, MO 63198
    Y.I. Otero, Asgrow Seed Co., Kalamazoo, MI 49002



Glyphosate (N-phosphonomethyl-glycine) is the active ingredient the nonselective herbicide Roundup. The sensitivity of crop plants to glyphosate has limited its in-season use as a postemergence herbicide. The extension of the use of Roundup herbicide to allow in-season application in major crops such as soybeans [Glycine max (L.) Merr.] would provide new weed control options for farmers. A glyphosate-tolerant soybean line, 40-3-2, was obtained through expression of the bacterial 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EPSPS) enzyme from Agrobacterium sp. strain CP4. Line 40-3-2 is highly tolerant to glyphosate, showing no visual injury after application of up to 1.68 kg acid equivalent (a.e.) ha−1 of glyphosate under field conditions. Molecular characterization studies determined that the single genetic insert in line 40-3-2 contains only a portion of the cauliflower mosaic virus 35S promoter (P-E35S), the Petunia hybrida EPSPS chloroplast transit peptide (CTP), the CP4 EPSPS gene, and a portion of the 3' nontranslated region of the nopaline synthase gene (NOS 3') terminator. Inheritance studies have shown that the transgene behaves as a single dominant gene and is stable over several generations.

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Copyright © 1995. Crop Science Society of America, Inc.Copyright © 1995 by the Crop Science Society of America, Inc.