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This article in CS

  1. Vol. 36 No. 6, p. 1676-1683
     
    Published: Nov, 1996


    * Corresponding author(s): marker@unity.ncsu.edu
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doi:10.2135/cropsci1996.0011183X003600060043x

Simple Sequence Repeat Markers Developed from Maize Sequences Found in the GENBANK Database: Map Construction

  1. M. L. Senior ,
  2. E. C. L. Chin,
  3. M. Lee,
  4. J. S. C. Smith and
  5. C. W. Stuber
  1. U SDA-ARS, Molecular Marker Facility, Box 7620, North Carolina State Univ., Raleigh NC 27695
    P ioneer HiBred International, Inc. 7300 N.W. 62nd Ave., Johnston IA 50131
    D ep. of Agronomy, Iowa State Univ., Ames IA 50011
    U SDA-ARS, Dep. of Genetics, Box 7614, North Carolina State Univ., Raleigh NC 27695

Abstract

Abstract

Simple sequence repeats (SSRs) are rapidly becoming an important class of DNA markers that are being widely used to map both plant and animal genomes. SSRs have the advantage of providing a codominant marker system based on polymerase chain reaction (PCR) methodology. Although the presence of SSRs is now well documented in the plant kingdom, a mapped set of primer sequences in maize (Zea mays L.) is not available. Polymorphic primer pairs developed from maize sequences in GENBANK were mapped to 42 loci in maize by means of either a B73 × Mo17, Mo17 × H99, or B73 × G35 recombinant inbred population. All SSR loci were found to be linked to one or more adjacent restriction fragment length polymorphism (RFLP) and/or isozyme loci. Segregation followed a pattern of Mendelian inheritance with one SSR locus deviating from expected ratios at a 1% level of significance. The SSRs were distributed throughout the maize genome with no evidence of clustering. Each SSR marker detected a single locus.

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