About Us | Help Videos | Contact Us | Subscriptions
 

Abstract

 

This article in CS

  1. Vol. 36 No. 6, p. 1706-1708
     
    Received: Dec 1, 1995
    Published: Nov, 1996


    * Corresponding author(s): hegstad@students.uiuc.edu
 View
 Download
 Alerts
 Permissions
 Share

doi:10.2135/cropsci1996.0011183X003600060048x

Pod Inoculation Technique with Phytophthora Sojae to Evaluate Soybean Populations for RPS Alleles in Field Plantings

  1. J. M. Hegstad ,
  2. D. E. Kyle and
  3. C. D. Nickell
  1. University of Illinois at Urbana-Champaign, Dep. of Crop Sciences, 1102 S. Goodwin, Urbana IL, 61801

Abstract

Abstract

The pathogen Phytophthora sojae (M.J. Kaufmann and J.W. Gerdemann) can be injected into soybean pods [Glycine max (L.) Merr.] determine presence of phytophthora root and stem rot resistance. The objective of this study was to use pod inoculation in the field to screen soybean F2 populations segregating for Rps alleles. The potential exists to inject different races of the pathogen into pods at different nodes of a single plant, allowing for multiple screenings at a time. Controlling the amount of Phytophthora sojae pathogen injected into each pod was necessary for accurate scoring of disease progression. Injecting 25 μL of Race 1 or Race 3 mycelium inoculum gave optimal symptom differences. The amount of disease progression up the pod to the point of attachment or the complete lack of rotting was determined to distinguish between susceptible and resistant plants, respectively. The data obtained from scoring pods conformed well to ratios of expected models. This technique provides a rapid and inexpensive field screening for identifying resistant or susceptible progeny early in the selection process.

Contribution from the Illinois Agric. Exp. Stn., Urbana IL. Research supported by the Illinois Soybean Program Operating Board and the Illinois Crop Improvements Asociation.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © .