Abstract

The monoclonal antibodies previously used to determine the role of ergot alkaloids in rescue toxicosis have higher affinity to iysergic acid amides than to ergopeptine alkaloids. The objective of this research was to find monoclonal antibodies with uniform affinity to all ergot alkaloid classes. Balb/C mice were immunized with ergonovine-protein conjugates. Splenic hybridoma cell lines were compared with current antibodies for cross reaction with lysergic acid amide and ergopeptine alkaloids. Antibodies from two cell lines had more uniform affinity to both ergot alkaloid classes and should be useful for determining the role of ergot alkaloids in fescue.

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