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This article in CS

  1. Vol. 37 No. 2, p. 537-543
     
    Received: Aug 2, 1996


    * Corresponding author(s): Paul.Keim@NAU.EDU
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doi:10.2135/cropsci1997.0011183X003700020038x

A High-Density Soybean Genetic Map Based on AFLP Markers

  1. Paul Keim ,
  2. James M. Schupp,
  3. Steven E. Travis,
  4. Kathryn Clayton,
  5. Tong Zhu,
  6. Liang Shi,
  7. Arnaldo Ferreira and
  8. David M. Webb
  1. Dep. of Biology Sciences, Northern Arizona Univ., Flagstaff AZ 86011-5640
    Dep. of Trait and Technology Development, Pioneer Hi-Bred, Int., Inc., P.O. Box 1004, Johnston, IA 50131
    Dep. of Trait and Technology Development, Pioneer Hi-Bred, Int., Inc., P.O. Box 1004, Johnston, IA 50131

Abstract

Abstract

Genetic marker systems have improved dramatically in the past 10 yr. Each new system needs to be evaluated for its distribution of markers on genetic linkage maps to validate its use for genetic analysis. The resulting maps are also useful for establishing the genetic positions of genes affecting important phenotypes. We have constructed a high density map in soybean [Glycine max (L.) Merr.] using a 300 RIL (recombinant inbred line) population from BSR-101 × PI437.654 by first constructing an RFLP (restriction fragment length polymorphism) “scaffold” map based on the entire population. The RFLP anchored map was then further populated with AFLP (amplified fragment length polymorphism) markers based on only a 42 RIL subset. We report here an 840 marker map consisting of 165 RFLP, 25 RAPD (random amplified polymorphic DNA), and 650 AFLP markers spread over 28 linkage groups representing 3441 cM distance. Although clustering of AFLP markers occurred, markers were mapped to every linkage group and were well distributed relative to other marker systems. The AFLP marker system appears to be a useful approach for generating high density genetic maps in soybean.

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