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This article in CS

  1. Vol. 37 No. 4, p. 1177-1180
     
    Received: Jan 12, 1996


    * Corresponding author(s): takeo-sa@po.iijnet.or.jp
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doi:10.2135/cropsci1997.0011183X003700040025x

Genetic Analysis of High Molecular Weight Proteins in Rice (Oryza sativa L.) Endosperm

  1. Gusti Ray Sadimantara,
  2. Toshinori Abe and
  3. Takeo Sasahara 
  1. Lab. of Plant Breeding, Faculty of Agriculture, Yamagata Univ., Tsuruoka 997, Japan

Abstract

Abstract

Genetic and physiological characteristics of the main rice (Oryza sativa L.) storage proteins, glutelin and prolamin, have been extensively studied. In contrast, information on high molecular weight proteins is limited. Experiments were undertaken to identify the genes that control the synthesis of high molecular weight proteins (40–90 kDa) in rice endosperm. Total endosperm proteins of japonica and indica cultivars, and their F2 progeny, were analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Nine high molecular weight proteins were detected in three cross combinations between japonica and indica cultivars. Segregation of these endosperm proteins in the F2 progeny fits with a ratio of 1:1:1:1 (HpxHpxHpx:HpxHpxhpx:Hpxhpxhpx:hpxhpxhpx), resulting from double fertilization. Three protein subunits (D, E, and M) were found only in the cross combination of Taichung 65 × BlueBelle, but other six protein subunits (F, G, H, I, K, and L) were commonly in three cross combinations. The dominant genes appeared to regulate expression of the high molecular weight protein subunits, anda dose effect of dominangt enes was detected.

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