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This article in CS

  1. Vol. 37 No. 4, p. 1370-1373
     
    Received: Sept 9, 1996


    * Corresponding author(s): stmartin+@osu.edu
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doi:10.2135/cropsci1997.0011183X003700040056x

Using AFLP Markers to Determine the Genomic Contribution of Parents to Populations

  1. Tara T. VanToai,
  2. Jiqing Peng and
  3. Steven K. St. Martin 
  1. U SDA-ARS, Soil Drainage Research Unit, 590 Woody Hayes Drive, Columbus, OH 43210
    C rops Res. Inst., Shandong Academy of Agricultural Sciences, Jinan, 250100, China
    D ep. of Horticulture and Crop Science, 202 Kottman Hall, 2021 Coffey Rd., Columbus, OH 43210-1086

Abstract

Abstract

Estimates of proportions of parental contribution to germplasm populations are useful to breeders. The objective of this research was to assess the ability of amplified fragment-length polymorphism (AFLP) markers to characterize the contributions of the original parents to later cycles of a soybean [Glycine max (L.) Merr.] population that had undergone recurrent selection. Use of four primer pairs yielded a total of 47 polymorphic bands. Estimates of parental contribution were consistent between primer pairs and between cycles and conformed to those expected based on pedigrees. Standard errors of estimates were 3 to 5%, indicating satisfactory precision. The AFLP markers should provide relatively inexpensive, precise estimates of parental contribution. This marker system may also be useful in tracking genetic changes in populations.

Some salaries and research support provided by state and federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State Univ. Journal Article No. 84–96.

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