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This article in CS

  1. Vol. 38 No. 2, p. 503-509
     
    Received: Mar 3, 1997


    * Corresponding author(s): pbeuselinck@psu.missouri.edu
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doi:10.2135/cropsci1998.0011183X003800020038x

Rhizomatous Lotus corniculatus L.: III. Introgression of Rhizomes into Autogamous Germplasm

  1. C. Nualsri,
  2. P. R. Beuselinck  and
  3. J. J. Steiner
  1. F aculty of Natural Sciences, Prince of Songkhla University, Hatyai, Songla (90112) Thailand, formerly Dep. of Agronomy, University of Missouri, Columbia, MO 65211
    U SDA-ARS, Plant Genetics Research Unit, Columbia, MO 65211
    U SDA-ARS, National Forage Seed Production Res. Center, Corvallis, OR. 97331

Abstract

Abstract

Introduction of genes for rhizomes from wild Lotus corniculatus L. germplasm into domesticated germplasm may be an important step towards improving persistence of cultivated L. corniculatus, normally a cross-pollinated herbage crop. This study was initiated to determine if rhizomes could be transferred to a L. corniculatus germplasm that sexually reproduces via autogamy. Autogamy is an unusual reproductive expression in this typically self-incompatible species. Compatibility was high between the autogamous germplasm AG-S4 and five wild Moroccan accessions with rhizomes. Higher crossing success was obtained when AG-S4 was used as a maternal parent. Most seeds produced were normal in appearance and size and had high germination. The resulting F1 progeny were morphologically intermediate between the parents for size and shape of leaf and floral characters, but appeared more similar to their Moroccan parents. Differences between reciprocal crosses were not evident. Hybridity of progeny was confirmed by random amplified polymorphic DNA (RAPD) analysis using eight primers. Fertility of F1 progeny was low relative to their parents. Meiosis of parental genotypes was characterized by a high frequency of bivalents, but the meiotic behavior of their F1 hybrids was more irregular. The frequency of univalents varied from 0 to 8 with an average 2.4 per ceil, indicating homology of chromosomes between AG-S4 and Moroccan accessions was not complete. Rhizomes were successfully expressed in F1 hybrids and a small number were found to also be autogamous. The autogamous hybrids will be important tools to elucidate the inheritance of rhizomes in L. corniculatus. Selection among the hybrids could lead to autogamous, rhizomatous cultivars of L. corniculatus.

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