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This article in CS

  1. Vol. 38 No. 3, p. 872-875
    Received: Mar 30, 1997

    * Corresponding author(s): jmosjidi@acesag.auburn.edu
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Rapid Prediction of Mating System of Vicia Species

  1. X. Zhang and
  2. J. A. Mosjidis 
  1. Dep. of Agronomy and Soils and Alabama Agric. Stn., Auburn Univ., AL 36849-5412, USA



Maintenance and use of germplasm collections of Vicia species is difficult because of limited information on their mating systems. Polymorphism of seven enzyme systems in 31 accessions of 12 Vicia species were measured by isoelectric focusing to infer their mating system. To compare variability within and among accessions and between species, polymorphism indices were calculated by means of isozyme bands. All enzymes studied had polymorphic banding patterns. Twenty-eight accessions were variable for at least one of the seven enzymes, whereas three other accessions were uniform for all seven enzymes. Within-accession variation was larger than that among accessions in V. villosa Roth and V. villosa ssp. varia (Host) Corbière, whereas within-accession variation was smaller than that among accessions of V. articulata Hornem., V. benghalensis L., V. cracca L., V. ervilia (L.) Willd., V. lutea L., V. narbonensis L., V. pannonica Crantz, V. peregrina L., and V. sativa L.. The results agreed with field tests which demonstrated that V. villosa and V. villosa ssp. varia are predominantly cross-fertilizing species, whereas the other species are mainly self-fertilizing. The use of isozyme bands to determine mating system proved to be accurate. In addition, this method is convenient because it utilizes a small seed sample and is inexpensive and easy to perform.

J. Series No. 3-975784.

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