About Us | Help Videos | Contact Us | Subscriptions
 

Abstract

 

This article in CS

  1. Vol. 38 No. 5, p. 1309-1319
     
    Received: Sept 15, 1997


    * Corresponding author(s): aghunt00@pop.uky.edu
 View
 Download
 Alerts
 Permissions
 Share

doi:10.2135/cropsci1998.0011183X003800050030x

The NIa-Proteinase of Different Plant Potyviruses Provides Specific Resistance to Viral Infection

  1. John P. Fellers,
  2. Glenn B. Collins and
  3. Arthur G. Hunt
  1. Dep. of Botany, North Carolina State Univ., Box 7612, Raleigh, NC 27695-7612

Abstract

Abstract

The genome-linked protein-proteinase (NIa) genes of three potyviruses (tobacco vein mottling virus[TVMV], tobacco etch virus[TEV], and potato virus Y [PVY]) were introduced into tobacco (Nicotiana tabacum L.) cultivar Burley 21 and transgenic plant lines were evaluated for viral symptoms and virus accumulation. Plant lines that expressed the TVMV NIa gene were resistant to infection by TVMV; inoculated plants did not develop disease symptoms after inoculation, and virus did not accumulate. Most lines that carried the TEV NIa and PVY NIa genes did develop symptoms after inoculation with TEV and PVY, respectively, but these symptoms abated with time (a response termed as recovery). Three genes, each consisting of two NIa coding regions (TEV NIa-PVY NIa, TEV NIa-TVMV NIa, and TVMV NIa-PVY NIa) were also introduced into Burley 21. Transgenic lines that carried either TEV NIa-PVY NIa or TEV NIa-TVMV NIa constructs either did not develop disease symptoms or exhibited the process of recovery after inoculation with TEV. TEV NIa-TVMV NIa and TVMV NIa-PVY NIa lines recovered from an initial infection after inoculation with TVMVL. Likewise, TEV NIa-PVY NIa and TVMV NIa-PVY NIa transformed lines recovered from an initial infection after inoculation with PVY. These results demonstrate that different potyvirus NIa-proteinase genes can be used for protection against virus infection and that multiple NIa genes may be used to produce plants that are protected against more than one potyvirus.

Contribution 97-06-147 of the Univ. of Kentucky Agric. Exp. Stn. Supported in part by USDA NRI grants #94-37303-0470 and #90-37262-5519

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © .