Nondestructive Assessment of Sinapic Acid Esters in Brassica Species: I. Analysis by Near Infrared Reflectance Spectroscopy
- Leonardo Velasco ,
- Bertrand Matthäus and
- Christian Möllers
Sinapic acid esters (SAE) are important antinutritional compounds of the seed of rapeseed (Brassica napus L.) and related Brassica species. The objective of this work was to study the potential of near infrared reflectance spectroscopy (NIRS) to analyze SAE content intact-seed samples of the genus Brassica. A total of 400 samples belonging to 17 species of Brassica were used to develop an initial calibration equation for this trait by using modified partial least squares regression on second derivative-transformed and scatter-corrected spectra. The r2 in cross validation was 0.91, and the standard error of cross validation was 1.1 g kg−1 seed (mean = 10.0). The calibration set was expanded with 187 further samples, selected for low SAE content with the initial equation, and the accuracy of the method was tested through both external and independent validation. In the first case, the analysis of samples not included but belonging to species represented in the calibration set led to an r2 of 0.92 and standard error of performance (SEP) of 1.1 g kg−1 (mean = 9.2). In the second case, the analysis of 57 samples of B. juncea (L.) Czern., not represented with any sample in the calibration, resulted in a SEP of 1.1 g kg−1 (mean = 7.3). The results demonstrated the robustness of the calibration equations to estimate SAE content in a wide range of species of the genus Brassica, even of those not used for calibration. NIRS discrimination of this trait was associated with eight spectral regions, all related to chemical absorbers in the sinapic acid molecule.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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