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Crop Science Abstract -

DNA-Based Markers for a Recessive Gene Conferring Anthracnose Resistance in Sorghum


This article in CS

  1. Vol. 38 No. 6, p. 1708-1709
    Received: Nov 23, 1997

    * Corresponding author(s): c-magill@tamu.edu
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  1. Khazan S. Boora,
  2. Richard Frederiksen and
  3. Clint Magill 
  1. Dep. of Biotechnology and Molecular Biology, Haryana Agric. Univ., Hisar 125004, India



Resistance to anthracnose in sorghum [Sorghum bicolor (L.) Moench] accession SC326-6 was found to segregate as a simple recessive trait in a cross to the susceptible cultivar BTx623.F3 progeny tests following self pollination of 115 F2 individuals identified homozygous resistant and susceptible F2 plants for use in bulked-segregant analysis. DNA from the parental cultivars and the bulks was screened by PCR amplification with 300 RAPD (random amplified polymorphic DNA) primers. Two RAPD primers amplified a sequence that co-segregated with the recessive resistance allele, while another amplified a band linked to the susceptible allele.

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