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This article in CS

  1. Vol. 38 No. 6, p. 1708-1709
     
    Received: Nov 23, 1997


    * Corresponding author(s): c-magill@tamu.edu
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doi:10.2135/cropsci1998.0011183X003800060048x

DNA-Based Markers for a Recessive Gene Conferring Anthracnose Resistance in Sorghum

  1. Khazan S. Boora,
  2. Richard Frederiksen and
  3. Clint Magill 
  1. Dep. of Biotechnology and Molecular Biology, Haryana Agric. Univ., Hisar 125004, India

Abstract

Abstract

Resistance to anthracnose in sorghum [Sorghum bicolor (L.) Moench] accession SC326-6 was found to segregate as a simple recessive trait in a cross to the susceptible cultivar BTx623.F3 progeny tests following self pollination of 115 F2 individuals identified homozygous resistant and susceptible F2 plants for use in bulked-segregant analysis. DNA from the parental cultivars and the bulks was screened by PCR amplification with 300 RAPD (random amplified polymorphic DNA) primers. Two RAPD primers amplified a sequence that co-segregated with the recessive resistance allele, while another amplified a band linked to the susceptible allele.

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