DNA-Based Markers for a Recessive Gene Conferring Anthracnose Resistance in Sorghum
- Khazan S. Boora,
- Richard Frederiksen and
- Clint Magill
Resistance to anthracnose in sorghum [Sorghum bicolor (L.) Moench] accession SC326-6 was found to segregate as a simple recessive trait in a cross to the susceptible cultivar BTx623.F3 progeny tests following self pollination of 115 F2 individuals identified homozygous resistant and susceptible F2 plants for use in bulked-segregant analysis. DNA from the parental cultivars and the bulks was screened by PCR amplification with 300 RAPD (random amplified polymorphic DNA) primers. Two RAPD primers amplified a sequence that co-segregated with the recessive resistance allele, while another amplified a band linked to the susceptible allele.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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