About Us | Help Videos | Contact Us | Subscriptions
 

Abstract

 

This article in CS

  1. Vol. 39 No. 1, p. 228-237
     
    Received: Mar 24, 1998


    * Corresponding author(s): melchinger@uni-hohenheim.de
 View
 Download
 Alerts
 Permissions
Request Permissions
 Share

doi:10.2135/cropsci1999.0011183X003900010035x

Genetic Similarities among Winter Wheat Cultivars Determined on the Basis of RFLPs, AFLPs, and SSRs and Their Use for Predicting Progeny Variance

  1. Martin Bohn,
  2. H. Friedrich Utz and
  3. Albrecht E. Melchinger 
  1. Institute of Plant Breeding, Seed Science, and Population Genetics, Univ. of Hohenheim, D-70593 Stuttgart, Germany

Abstract

Abstract

The efficiency of breeding programs could be increased by predicting the prospects of crosses for line development before producing and testing lines derived from them. In this study, we (i) assessed the level of genetic diversity among German and Austrian winter wheat (Triticum aestivum L.) cultivars using 117 restriction fragment length polymorphism (RFLP) probes, 16 amplified fragment length polymorphism (AFLP) primer combinations, and 21 simple sequence repeat (SSR) primer pairs, (ii) investigated the correlation between coancestry (f) and genetic similarity (GS) estimated from molecular markers, and (iii) evaluated the use of f and GS for predicting the genetic variance (σ2g) within sets of 22 F4n (n = 7 or 8) lines derived from 30 crosses between these winter wheat cultivars. The average polymorphic information content (PIC) for polymorphic bands was not significantly different between the three marker systems (0.30 ≤ PIC ≤ 0.33), whereas the marker index was low for RFLPs and SSRs but high for AFLPs. Estimates of f between all 55 cultivar combinations ranged from 0.01 to 0.53. -values varied between 0.52 and 0.89 for RFLPs, between 0.40 and 0.83 for AFLPs, and between 0.16 and 0.91 for SSRs. The Mantel Z test statistic revealed no common pattern between the four dendrograms obtained by cluster analyses. Significant (P < 0.05) correlations among , -RFLP, -AFLP, and -SSR were detected only for related parent combinations (≥ 0.10). For all seven traits analyzed, estimates of σ2g were not significantly associated with any measure of GS between parents. On the basis of these results, we recommend AFLPs for fingerprinting wheat cultivars. However, predicting the progeny variance (σ2g) remains an unsolved problem.

 
 
 
 
  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © .