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Isolation of High Seed Inorganic P, Low-Phytate Soybean Mutants


This article in CS

  1. Vol. 40 No. 6, p. 1601-1605
    Received: Aug 30, 1999

    * Corresponding author(s): jwilcox@purdue.edu
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  1. James R. Wilcox *a,
  2. Gnanasiri S. Premachandrab,
  3. Kevin A. Youngc and
  4. Victor Raboyc
  1. a USDA-ARS, Crop Production and Pathology Research and Dep. of Agronomy, Purdue Univ., West Lafayette, IN 47907-1150 USA
    b Dep. of Agronomy, Purdue Univ., Aberdeen, ID USA
    c USDA-ARS, Small Grain and Potato Germplasm Research, 1691 South 2700 West, Aberdeen, ID 83210 USA


Phosphorous in soybean [Glycine max (L.) Merr.] seed is stored primarily as phytic acid, which is nutritionally unavailable to nonruminant livestock. The objective of this study was to isolate mutations that reduce soybean seed phytic acid P and increase seed inorganic P. Following treatment with ethyl methanesulfonate, M2 through M6 plants were screened for high seed inorganic P. Seeds of M2 plants high in inorganic P produced progenies high in inorganic P through the M6 generation. M6 progenies of one plant averaged 6.84 g kg−1 seed phytic acid and inorganic P varied from 2.34 to 4.41 g kg−1 or 60 to 66% of phytic acid P plus inorganic P. M6 progenies of a second plant averaged 10.89 g kg−1 phytic acid and varied from 1.21 to 3.84 g kg−1 inorganic P, representing from 47 to 51% of the sum of phytic acid P plus inorganic P. In contrast, nonmutant seeds of the check cultivar Athow contained 15.33 g kg−1 phytic acid and averaged 0.74 g kg−1 inorganic P, representing 15% of the sum of phytic acid P plus inorganic P. Low phytic acid and high inorganic P in these progenies should increase the nutritional value of soy meal and reduce excess P in livestock manure.

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