Mapping and Progress toward Map-Based Cloning of Brown Planthopper Biotype-4 Resistance Gene Introgressed from Oryza officinalis into Cultivated Rice, O. sativa
- K. Renganayaki *a,
- Allan K. Fritzb,
- S. Sadasivame,
- Sujata Pammic,
- Sandra E. Harringtond,
- Susan R. McCouchd,
- S. Mohan Kumare and
- Avutu Sam Reddyc
- a Dep. of Soil and Crop Sciences, Texas A&M Univ., College Station, TX 77843
b Dep. of Agronomy, Kansas State Univ., Manhattan, KS 66506
e Centre for Plant Molecular Biology, Tamil Nadu Agricultural Univ., Coimbatore 641 003, India
c Dow Agro Sciences, 9330 Zionsville Road, Indianapolis, IN 46268-1054
d Dep. of Plant Breeding and Biometry, Cornell Univ., Ithaca, NY 14853-1901
Brown plant hopper (BPH), Nilaparvata lugens (Stal.), is a serious insect pest of rice in Asia, causing direct losses and vectoring Rice grassy stunt virus (RGSV) and Rice ragged stunt virus (RRSV). Recombinant inbred lines (RILs) developed from a cross between ‘IR50’ and ‘IR54745-2-21-12-17-6’ were used to identify random amplified polymorphic DNA (RAPD) markers closely linked to a BPH Biotype-4 resistance gene [Bph13 (t)] derived from Oryza officinalis Wall. Bulked segregant analysis (BSA) using RAPD primers identified 11 polymorphic fragments. Six fragments, AJ09260a, AL05220a, AK10690a, AK10430c, AK10380d, and AJ01200a, were linked in coupling phase to the Bph13 (t) locus. The remaining five fragments, AJ09230b, AJ09180c, AJ09100d, AL05400b, and AK10340e, were linked in repulsion. The most closely linked RAPD marker, AJ09230b, was converted to a codominant linked sequence tagged sites (STS) marker. This marker mapped 1.3 centimorgans (cM) from the resistance gene and was placed on rice chromosome 3 by means of ‘IR64’ × ‘Azucena’ doubled haploid (DH) population. The tightly linked STS marker could be used for marker-assisted selection (MAS). In addition, these markers will be useful for a positional cloning strategy to isolate the resistance gene.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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