Developing Midstearic Acid Sunflower Lines from a High Stearic Acid Mutant
- Begoña Pérez-Vich *,
- Juan Muñoz-Ruz and
- José M. Fernández-Martínez
Sunflower (Helianthus annuus L.) genotypes with increased stearic acid (C18:0) content in their seed oil may be useful for food and industrial applications. The objective of this study was to isolate and characterize mid-stearic acid sunflower lines homozygous recessive for single genes from the high stearic acid mutant CAS-3 (250 g kg−1). Crosses between CAS-3 (genotype es1es1es2es2), and the inbred line HA 89, with standard low stearic acid levels (50 g kg−1; Es1Es1Es2Es2), were made to obtain F3 families segregating for either the Es1 or Es2 loci. From these families, F3 half-seeds with putative genotypes es1es1Es2Es2 (188 g kg−1) and Es1Es1es2es2 (90 g kg−1) were selected. F7–lines homozygous for es1 or es2 were developed. These lines were named CAS-19 (es1es1Es2Es2) and CAS-20 (Es1Es1es2es2) and showed mid-stearic acid levels of 168 g kg−1 (CAS-19) and 83 g kg−1 (CAS-20). The fatty acid content was evaluated in all generations by gas-liquid chromatography. The genetic composition of CAS-19 and CAS-20 was verified by evaluating progenies from crosses between both mid-stearic acid lines, and from crosses between the mid-stearic acid lines and HA 89. The new Es1Es1es2es2 and es1es1Es2Es2 genotypes expressing mid-stearic acid levels represent a further advance for the development of sunflower lines for specific edible purposes, and constitute a unique source for agronomic and genetic studies on single alleles controlling increased stearic acid content in sunflower.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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