About Us | Help Videos | Contact Us | Subscriptions
 

Abstract

 

This article in CS

  1. Vol. 44 No. 2, p. 418-424
     
    Received: May 1, 2003
    Published: Mar, 2004


    * Corresponding author(s): esouza@uidaho.edu
 View
 Download
 Alerts
 Permissions
 Share

doi:10.2135/cropsci2004.4180

Identification and Characterization of a Low Phytic Acid Wheat

  1. Mary Guttieria,
  2. David Bowena,
  3. John A. Dorschb,
  4. Victor Raboyc and
  5. Edward Souza *a
  1. a Univ. of Idaho, Aberdeen Research and Extension Center, P.O. Box 870, Aberdeen, ID 83210
    b BASF Corp., Research Triangle Park, NC 27709
    c USDA-ARS Small Grains and Potato Research Unit, P.O. Box 607, Aberdeen, ID 83210

Abstract

Phytic acid (myo-inositol-1,2,3,4,5,6-hexakisphosphate, or Ins P6) is the most abundant storage form of P in seeds, yet indigestible by humans and nonruminant livestock. A wheat (Triticum aestivum L.) mutant is described herein with greatly reduced seed phytic acid P but little change in seed total P, similar to lpa1-type mutants described in other grain species. One nonlethal mutant from 562 ethyl-methanesulfonate (EMS) mutagenized M2 lines was identified with a high inorganic phosphate (HIP) phenotype and designated Js-12-LPA. Js-12-LPA homozygotes produced seed in which phytic acid P represented 48.2% of seed total P, in contrast to 74.7% of seed total P in nonmutant or wild-type control, Js-12-WT. The inorganic portion of seed P was increased from 9.1% in Js-12-WT to 50.1% in Js-12-LPA, with little effect on total seed P. Weight distributions among milling fractions were similar for the Js-12-LPA and Js-12-WT genotypes. The low phytic acid trait altered the distribution of total P within the kernel, increasing the P content of the central endosperm and decreasing the P content of the bran. The low phytic acid trait decreased the phytic acid concentration in the bran by 43% and increased the inorganic P concentration in the bran nearly four-fold. Inheritance data of F2 and F4:6 families was inconsistent with a single-gene mutation and suggests the involvement of two or more genes. This low phytic acid wheat mutant is a genetic resource for studying the biology of seed phytic acid metabolism and wheat quality improvement.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © 2004. Crop Science Society of AmericaCrop Science Society of America