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The 4E-ms System of Producing Hybrid Wheat


This article in CS

  1. Vol. 46 No. 1, p. 250-255
    Received: Jan 11, 2005

    * Corresponding author(s): zhoukuanji@sohu.com
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  1. Kuanji Zhou *ab,
  2. Shihong Wangb,
  3. Yuqin Fengb,
  4. Zhongxiang Liub and
  5. Genxuan Wanga
  1. a State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou, Gansu, China.730070
    b Food Crops Institute, Gansu Academy of Agricultural Science, Lanzhou, Gansu, China.730070


A cytogenetic method for producing hybrid seed using a nuclear gene for male sterility and an alien chromosome to obtain a pollination control system has been proposed in bread wheat (Triticum aestivum L.). Our objective was to transfer the alien chromosome 4E to the monogenic recessive male-sterile mutant Lanzhou (LZ), and then to establish an efficient cytogenetic system of maintaining the male sterility of the LZ mutant. After crossing the 4E disomic addition line 81529 (2n = 44) as male to LZ [2n = 42(msms) = 42], we obtained the 4E monosomic addition line [2n = 43(msms)] in the F3 generation that had light-blue seed color. The line was homozygous for the male-sterile gene (msms) of the host wheat and also had good self-fertility. The self-fertilized seeds segregated into 64.3% white (nonblue) with genotype 2n = 42(msms), 32.1% light blue with genotype 2n = 43(msms) and 3.6% deep blue with genotype 2n = 44(msms) grains. All plants grown from the white grains were completely male sterile. All plants grown from the light-blue grains had good self-fertility whose progeny seeds segregated into white, light-blue, and deep-blue grains again in succeeding generations. All plants grown from the deep-blue grains were self-fertile and retained their deep-blue color in the succeeding generations. When white grain male-sterile lines were crossed using any cultivar as the male parent, the hybrid plants had male fertility restored. And, this is the basis for the 4E-ms system of producing hybrid wheat.

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