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This article in CS

  1. Vol. 46 No. 1, p. 362-364
    Received: June 22, 2005

    * Corresponding author(s): jgoldman@spa.ars.usda.gov
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High-Throughput DNA Extraction and Allele Specific PCR Primers Enables Efficient Screening for Mutant (gsf) and Wild-Type (GSF) Alleles in Eastern Gamagrass

  1. Jason J. Goldman *
  1. USDA-ARS, Southern Plains Range Res. Stn., 2000 18th St., Woodward, OK 73801


Eastern gamagrass [Tripsacum dactyloides (L.) L.], is a perennial warm-season bunchgrass with high forage potential if properly managed. Low seed production is one factor that has limited wide spread use of this native grass. A recessive gynomonoecious sex form (GSF) mutation previously found in a wild population has the potential to increase seed production, although it may also be linked to other deleterious traits. Allele specific primers were designed to detect the wild-type (GSF) and mutant (gsf) allele. Primers were successful at confirming the genotype of an assortment of plants tested using template DNA from a high-throughput extraction protocol. A tissue-pooling strategy enabled detection of the mutant allele when present in a predominantly wild-type background. The low cost, lack of toxic waste, and speed of the DNA extraction protocol and the ability to efficiently genotype a large number of individuals makes this a practical tool in a breeding program working with the GSF trait.

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Copyright © 2006. Crop Science Society of AmericaCrop Science Society of America