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Fine Mapping of a Major Insect Resistance QTL in Soybean and its Interaction with Minor Resistance QTLs


This article in CS

  1. Vol. 46 No. 3, p. 1094-1099
    Received: June 7, 2005

    * Corresponding author(s): wparrott@uga.edu
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  1. S. Zhua,
  2. D. R. Walkera,
  3. H. R. Boermaa,
  4. J. N. Allb and
  5. W. A. Parrott *a
  1. a Center for Applied Genetic Technologies and Dep. of Crop & Soil Sciences
    b Dep. of Entomology, Univ. of Georgia, Athens, GA 30602


Utilization of native insect resistance genes can be an important component for managing insects in soybean [Glycine max (L.) Merr.]. A major quantitative trait locus (QTL-M) for insect resistance from PI 229358, controlling antibiosis and antixenosis, was previously identified on linkage group (LG) M and was found to increase the effectiveness of a Bacillus thuringiensis (Bt) transgene in soybean. The objectives of this study were to fine-map QTL-M using recombinant substitution lines (RSLs) identified from a ‘Benning’ backcross population, and to evaluate the main effects and the epistatic interactions between QTL-M and other resistance QTLs on LGs G and H using near-isogenic lines (NILs) in a Benning genetic background. The effect of QTL-M was still detectable in the Benning NILs when they were evaluated for resistance to corn earworm [CEW, Helicoverpa zea (Boddie)]. The two minor resistance QTLs only provided insect resistance when QTL-M was also present in the Benning NILs. The QTL-M was fine-mapped to an approximately 0.52-cM region after the first round of phenotyping the RSLs for resistance to CEW and soybean looper [SBL, Pseudoplusia includens (Walker)]. These results should increase the feasibility of cloning QTL-M and help guide the development of insect resistant soybean cultivars.

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Copyright © 2006. Crop Science Society of AmericaCrop Science Society of America