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Crop Science Abstract - CROP BREEDING & GENETICS

SSR Mapping and Confirmation of Soybean QTL from PI 437654 Conditioning Resistance to Reniform Nematode


This article in CS

  1. Vol. 47 No. 4, p. 1336-1343
    Received: Oct 9, 2006

    * Corresponding author(s): ha@uga.edu
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  1. Bo-Keun Ha *a,
  2. Robert T. Robbinsb,
  3. Feng Hanc,
  4. Richard S. Husseyd,
  5. John F. Soperc and
  6. H. Roger Boermaa
  1. a Dep. of Crop and Soil Science, Univ. of Georgia, Center for Applied Genetic Technologies, 111 Riverbend Rd., Athens, GA 30602
    b Dep. of Plant Pathology, Univ. of Arkansas, Fayetteville, AR 72701
    c Pioneer Hi-Bred International, Inc., Johnston, IA 50131
    d Dep. of Plant Pathology, Univ. of Georgia, Athens, GA 30602


Recently, reniform nematode (Rotylenchulus reniformis Linford and Oliveira) has been recognized to be a major problem on soybean [Glycine max (L.) Merr.] in the southern USA. Planting reniform nematode–resistant soybean cultivars is the most cost effective control method. With restriction fragment length polymorphism (RFLP) markers, Pioneer Hi-Bred International, Inc. had previously identified quantitative trait loci (QTL) on linkage group B1 (LG-B1) and another on LG-L associated with reniform nematode resistance in a cross between ‘BSR101’ and PI 437654. In this study, we refined the QTL locations and identified additional QTL conditioning resistance to reniform nematode in a population of 228 recombinant inbred lines (RILs) from a cross of BSR101 × PI 437654. A QTL (R 2 = 21%) conditioning reniform reproductive index (RI) was found on LG-L and was flanked by Sat_184 and Satt513. Two other QTL were identified, one on LG-B1 (R 2 = 16%) and the other on LG-G (R 2 = 8%). These two QTL acted in an epistatic manner with lines homozygous for PI 437654 alleles at both QTL providing the lowest reniform RI. The 31 RILs from BSR101 × PI 437654 that were homozygous for the PI 437654 alleles at Satt513, Satt359, and Sat_168 averaged 1077 reniform eggs and juveniles (RI = 0.63), while the 33 RILs homozygous for the BSR101 alleles averaged 10606 (RI = 6.24). By screening the RILs population of ‘Prichard’ × ‘Anand’ we confirmed that the QTL on LG-G and-B1 conditioned reniform RI.

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