About Us | Help Videos | Contact Us | Subscriptions

Members of ASA, CSSA, and SSSA: Due to system upgrades, your subscriptions in the digital library will be unavailable from May 15th to May 22nd. We apologize for any inconvenience this may cause, and thank you for your patience. If you have any questions, please call our membership department at 608-273-8080.


Institutional Subscribers: Institutional subscription access will not be interrupted for existing subscribers who have access via IP authentication, though new subscriptions or changes will not be available during the upgrade period. For questions, please email us at: queries@dl.sciencesocieties.org or call Danielle Lynch: 608-268-4976.



This article in CS

  1. Vol. 47 No. 4, p. 1691-1697
    Received: Nov 6, 2006

    * Corresponding author(s): faltpeter@ifas.ufl.edu
Request Permissions


Apomictic Bahiagrass Expressing the bar Gene Is Highly Resistant to Glufosinate under Field Conditions

  1. Sukhpreet Sandhua,
  2. Fredy Altpeter *a and
  3. Ann R. Blountb
  1. a Agronomy Dep., Plant Molecular and Cellular Biology Program, Genetics Institute, Univ. of Florida-IFAS, Gainesville, FL 32611
    b NFREC, Agronomy Dep., Univ. of Florida-IFAS, Marianna, FL


Bahiagrass (Paspalum notatum Flugge) is one of the most important low-input turf and forage grasses in the southeastern USA and in other subtropical regions. Its open growth habit, however, facilitates weed encroachment and its low tolerance to commercially available herbicides complicates weed management. Genetic transformation protocols were recently developed for bahiagrass that allow now, among other approaches, the introduction of herbicide resistance genes. Integration of the bar gene expression cassette into the genomic DNA of the apomictic bahiagrass cultivar Argentine was confirmed by Southern blot analysis. Stable expression of the bar gene was detected by an immunochromatographic assay in primary transgenic lines and the seed-derived progeny plants. Several independent transgenic lines showed no growth inhibition, chlorosis, or necrosis following a spray application of 1.0% glufosinate ammonium [2-amino-4-(hydroxymethylphosphinyl)butanoic acid] under greenhouse conditions. The selected transgenic plants did not differ morphologically from wild-type plants and produced viable seeds in the greenhouse and field. All weeds that coestablished during the field experiment, as well as wild-type bahiagrass, displayed full leaf necrosis after application of 0.3% glufosinate ammonium. Transgenic bahiagrass plants were resistant to a field application of 0.6% glufosinate ammonium, which is twice the recommended rate for weed control, without any symptoms of phytotoxicity.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © 2007. Crop Science Society of AmericaCrop Science Society of America