About Us | Help Videos | Contact Us | Subscriptions
 

Abstract

 

This article in CS

  1. Vol. 50 No. 3, p. 1037-1047
     
    Received: June 4, 2009


    * Corresponding author(s): mdacosta@psis.umass.edu
 View
 Download
 Alerts
 Permissions
Request Permissions
 Share

doi:10.2135/cropsci2009.06.0293

Physiological Changes during Cold Acclimation of Perennial Ryegrass Accessions Differing in Freeze Tolerance

  1. Lindsey Hoffmana,
  2. Michelle DaCosta *a,
  3. J. Scott Ebdona and
  4. Eric Watkinsb
  1. a Dep. of Plant, Soil, and Insect Sciences, Univ. of Massachusetts, 80 Campus Center Way, Amherst, MA 01003
    b Dep. of Horticultural Science, Univ. of Minnesota, 1970 Folwell Ave., St. Paul, MN 55108

Abstract

Perennial ryegrass (Lolium perenne L.) is a cool-season turfgrass species that can exhibit significant freezing injury in northern climatic regions. The objective of this study was to quantify major physiological and biochemical changes occurring during the cold acclimation period in four perennial ryegrass accessions varying in freezing tolerance (two freezing-tolerant accessions, TOL-1 and TOL-2, and two freezing-susceptible accessions, SUS-1 and SUS-2). Plants were subjected to a cold acclimation period of 2°C for 21 d in a controlled environment chamber. Crown tissues were harvested at 0, 7, 14, and 21 d of acclimation for determination of proline, water soluble carbohydrates (WSC), and lipid composition. Leaf photochemical efficiency (Fv/Fm) was also measured weekly before plant sampling for biochemical components. In general, proline levels increased and Fv/Fm decreased during cold acclimation; however, there were no differences in proline accumulation or Fv/Fm among the accessions in response to cold acclimation. Water soluble carbohydrates significantly increased in response to 2°C, with TOL accessions (TOL-1 and TOL-2) accumulating higher WSC than SUS (SUS-1 and SUS-2) by 21 d of acclimation. There were also significant changes in major phospholipids classes at 2°C. Specifically, TOL-2 exhibited a higher ratio of membrane stabilizing lipids and unsaturated fatty acid content compared to SUS-1, SUS-2, and TOL-1 by 21 d of cold acclimation.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © 2010. Crop Science Society of AmericaCrop Science Society of America