About Us | Help Videos | Contact Us | Subscriptions
 

Abstract

 

This article in CS

  1. Vol. 50 No. 4, p. 1207-1218
     
    Received: Oct 27, 2009


    * Corresponding author(s): nick.tinker@agr.gc.ca
 View
 Download
 Alerts
 Permissions
Request Permissions
 Share

doi:10.2135/cropsci2009.09.0474

A Set of New Simple Sequence Repeat and Avenin DNA Markers Suitable for Mapping and Fingerprinting Studies in Oat (Avena spp.)

  1. Charlene P. Wighta,
  2. Weikai Yana,
  3. Jennifer Mitchell Fetchb,
  4. Jitka Deyla and
  5. Nicholas A. Tinker *a
  1. a Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Central Experimental Farm, 960 Carling Ave., Ottawa, ON K1A 0C6, Canada
    b Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Rd., Winnipeg, MB R3T 2M9, Canada. ECORC contribution number: 09-028

Abstract

This study was conducted to develop and test new markers for use in molecular characterization of oat (Avena spp.). Results are presented for two polymerase chain reaction (PCR)-based markers targeting multiple avenin protein loci and 16 new microsatellite or simple sequence repeat (SSR) markers. The markers were mapped in two segregating oat (Avena sativa L.) populations, ‘Kanota’ × ‘Ogle’ (K×O) and ‘Terra’ × ‘Marion’ (T×M), and were also tested across a diversity panel consisting of 35 cultivated varieties of oat and representatives of five other Avena species. All markers were robust, multiallelic, and highly polymorphic. The SSR markers, some highlighting multiple loci, mapped throughout the genome, and the avenin markers mapped to their expected locations in K×O and T×M. Also as expected, the avenin markers were shown to be linked to the stem rust resistance gene Pg9 in the population ‘OT328’ × ‘Dumont’ (OT×Du). Practical applications in variety identification and germplasm diagnostic assays were explored. The markers were able to distinguish closely related lines for fingerprinting purposes and were also useful for determining whether variation within a line was the result of residual heterozygosity or contamination. In addition, the markers were found to be useful for forensic purposes and for determining whether crossing and backcrossing had been successful.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © 2010. Crop Science Society of AmericaCrop Science Society of America