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Molecular and Cytogenetic Characterization of Wheat Introgression Lines Carrying the Stem Rust Resistance Gene Sr39


This article in CS

  1. Vol. 50 No. 4, p. 1393-1400
    Received: Oct 7, 2009

    * Corresponding author(s): Daryl.Klindworth@ars.usda.gov
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  1. Guo Tai Yua,
  2. Qijun Zhangb,
  3. Daryl L. Klindworth *c,
  4. Timothy L. Friesenc,
  5. Ron Knoxd,
  6. Yue Jine,
  7. Shaobin Zhongf,
  8. Xiwen Caib and
  9. Steven S. Xuc
  1. a Department of Entomology, North Dakota State University, Fargo, ND 58105
    b Department of Plant Sciences, North Dakota State University, Fargo, ND 58105
    c USDA-ARS Cereal Crops Research Unit, Box 5677, State University Station, Fargo, ND 58105-5677
    d AAFC SPARC, P.O. Box 1030, Swift Current, SK, S9H 3X2
    e USDA-ARS, Cereal Disease Laboratory, University of Minnesota, St. Paul, MN 55108
    f Department of Plant Pathology, North Dakota State University, Fargo, ND 58105. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture


The stem rust (Puccinia graminis Pers.:Pers. f.sp. tritici Eriks. and Henn.) resistance gene Sr39, which confers resistance to TTKSK (Ug99), has been incorporated into the wheat (Triticum aestivum L.) genome from Aegilops speltoides in the form of a chromosome translocation but it has not been deployed into adapted cultivars. In this study, we characterized translocation lines carrying Sr39 in four different wheat backgrounds with fluorescent genomic in situ hybridization (GISH) and simple sequence repeat (SSR) markers. The results indicated that RL5711 and RL6082 had translocation chromosomes of comparable structure. The translocation chromosome in PI 600683 has lost Ae. speltoides chromatin in the telomeric end of the 2S long arm. Six translocation lines derived from the cross PI 600683/3*HY438 had translocation chromosomes of comparable structure to the one found in PI 600683. However, one line (P9714-AM03C51), showed a substantial reduction in Ae. speltoides chromatin in the short arm of the translocated chromosome. The study demonstrated that it is apparently feasible to shorten Ae. speltoides chromatin in some wheat-Ae. speltoides translocation lines. These results and the identification of diagnostic SSR markers will be useful in guiding chromosome manipulation efforts to further shorten the Ae. speltoides chromosome segments in these materials. Greenhouse inoculation of translocation lines with stem rust indicated that the Sr39 gene conditions resistance to at least seven stem rust races.

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