Diallelic Nuclear Microsatellites for Diversity and Population Analyses of the Allotetraploid Creeping Bentgrass (Agrostis stolonifera)
- Jay R. Reichman *a,
- Bonnie M. Smitha,
- Jason P. Londob,
- Michael A. Bollmana,
- Carol A. Auerc and
- Lidia S. Watruda
- a Western Ecology Division, U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Lab., 200 SW 35th St., Corvallis, OR 97333
b National Research Council Associate, 200 SW 35th St., Corvallis, OR 97333
c Dep. of Plant Science, Univ. of Connecticut, Storrs, CT 06269. This work was funded by the U.S. Environmental Protection Agency
Nine diallelic nuclear microsatellite loci were characterized and developed as markers for analyses of diversity and population biology in creeping bentgrass, Agrostis stolonifera L. (2n = 4x = 28, A2A2A3A3). They may also help with identification of bentgrass germplasm resources. Polymorphic loci were isolated from genomic libraries from A. stolonifera and from A. transcaspica Litv. (2n = 2x = 14, putative A3A3). Markers were characterized in 87 A. stolonifera individuals from six distinct population sources. Analysis of molecular variance indicated significant variation between crop and wild groups. Highly significant variation was observed among and within the six populations. Bayesian analyses with STRUCTURE resolved crop from wild genotypes and assigned individuals to clusters that corresponded to their sources, in agreement with principal coordinates analysis results. The markers also correctly identified intraspecific creeping bentgrass F1 hybrids. Cross-species analyses were conducted on sexually compatible relatives A. gigantea R. (2n = 6x = 42, A1A1A2A2A3A3), A. capillaris L. (2n = 4x = 28, A1A1A2A2), and A. canina L. (2n = 2x = 14, A1A1 or possibly A2A2), plus A. transcaspica Seven markers were diallelic in A. gigantea Six of these markers were also diallelic in A. transcaspica, but not other species, suggesting that these loci are A3 genome specific. Results also support the hypothesis that A. transcaspica is the source of the A3 subgenome found in A. stolonifera and A. giganteaPlease view the pdf by using the Full Text (PDF) link under 'View' to the left.
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