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Crop Science Abstract - Crop Breeding & Genetics

Allelic Variants at the TaZds-D1 Locus on Wheat Chromosome 2DL and their Association with Yellow Pigment Content


This article in CS

  1. Vol. 51 No. 4, p. 1580-1590
    Received: Dec 9, 2010

    * Corresponding author(s): xiaxianchun@caas.net.cn
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  1. Caiying Zhanga,
  2. Changhai Dongab,
  3. Xinyao Heb,
  4. Liping Zhangbc,
  5. Xianchun Xia *b and
  6. Zhonghu He *bd
  1. a North China Key Lab. for Crop Germplasm Resources of Educational Ministry, Agricultural Univ. of Hebei, 289 Lingyusi St., Baoding 071001, China
    b Institute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South St., Beijing 100081, China
    c Beijing Engineering and Technique Research Center of Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China
    d International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South St., Beijing 100081, China


Zeta (ζ)-carotene desaturase (ZDS) is a key enzyme in the carotenoid biosynthetic pathway, which determines the yellow pigment (YP) content in wheat (Triticum aestivum L.) grains. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2DL, designated TaZds-D1, was characterized. The TaZds-D1 gene has an open reading frame of 1707 bp, including 13 introns and 14 exons, and a deduced peptide containing 568 amino acid residues with a predicted molecular weight of 62.5 kDa. The TaZds-D1 gene was located on chromosome 2DL based on polymerase chain reaction amplifications of Chinese Spring nullisomic-tetrasomic lines, Langdon–Chinese Spring D-genome chromosome disomic substitution lines, and Rusty D-genome chromosome disomic substitution lines as well as linkage analysis. Two allelic variants, TaZds-D1a and TaZds-D1b, were detected in Chinese wheat cultivars, and a functional marker, YP2D-1, was developed based on the sequence polymorphism between TaZds-D1a and TaZds-D1b. A major quantitative trait locus, located at the TaZds-D1 region in the marker interval of YP2D-1 and Xgwm157 on chromosome 2DL, explained 18.4% of the phenotypic variance for YP content in a doubled haploid population derived from the cross Zhongyou 9507/CA 9632. This confirmed the association between allelic variation at the TaZds-D1 locus and YP content.

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