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This article in CS

  1. Vol. 51 No. 5, p. 2119-2124
     
    Received: Nov 15, 2010


    * Corresponding author(s): wujiahe@im.ac.cn
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doi:10.2135/cropsci2010.11.0650

Transgenic Potato Overexpressing the Amaranthus caudatus Agglutinin Gene to Confer Aphid Resistance

  1. Yang Xina,
  2. Zhang Xiangronga,
  3. Zhang Mingjuab,
  4. Guo Wenchaoa,
  5. Tian Yingchuana,
  6. Xia Qizhongab and
  7. Wu Jiahe *a
  1. a State Key Lab. of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101China
    b now working in Huanggang Normal Univ

Abstract

Potato (Solanum tuberosum L.) was transformed with a pBCACAc vector harboring the Amaranthus caudatus agglutinin (ACA) gene mediated by Agrobacterium tumefaciens (Smith and Townsend 1907) Conn. One hundred and eighteen of a total 320 regenerated plants were confirmed to possess the ACA gene integrated into the potato genome as detected by polymerase chain reaction (PCR) and Southern blot analysis. Three transgenic events (referred to as PA1, PA2, and PA3) with one copy of the ACA gene insertion and no obvious phenotypic change were selected and further evaluated for expression level of ACA protein and performance in insect bioassays. Based on western blot assays, the content of ACA protein in PA1, PA2, and PA3 events was high at 0.11, 0.32, and 0.29%, respectively, of total soluble leaf protein. The propagation of peach-potato aphids (Myzus persicae Sulzer) in the three events was significantly inhibited, with 49, 18.5, and 25 aphids per plant, respectively, compared to 102.5 aphids per plant in nontransformed (NT) controls based on insect bioassays in greenhouse trials. The results of a field trial (in Beijing in 2009) showed that the number of aphids was significantly lower in transgenic plants than in NT. Tuber weight and plant yield of the three transgenic events were significantly higher than those of NT. Our results indicated that PA2 and PA3 can be regarded as potential germplasm to breed new cultivars that may decrease production loss from resistance to aphids and reduced indirect virus damage.

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