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Crop Science Abstract - Crop Breeding & Genetics

Genetic Characterization and Molecular Mapping Pse-2 Gene for Resistance to Halo Blight in Common Bean


This article in CS

  1. Vol. 51 No. 6, p. 2439-2448
    Received: Jan 25, 2011

    * Corresponding author(s): phil.miklas@ars.usda.gov
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  1. Phillip N. Miklas *a,
  2. Deidré Fourieb,
  3. Jennifer Trappa,
  4. Richard C. Larsena,
  5. Carolina Chavarroc,
  6. Matthew W. Blairc and
  7. Paul Geptsd
  1. a USDA-ARS, Vegetable and Forage Crop Research Unit, 24106 N. Bunn Rd., Prosser, WA 99350
    b ARC Grain Crops Institute, Potchefstroom, South Africa
    c CIAT, Apartado Aereo 6713, Cali, Columbia; and
    d Univ. of California, Dep. of Plant Sciences/MS1, 1 Shields Ave., Davis, CA 95616


Pseudomonas syringae pv. phaseolicola (Burkholder) Young et al. (Psp) causes halo blight, which is a serious bacterial disease of common bean (Phaseolus vulgaris L.). Several resistance (R) genes have been discovered in host differential cultivar ZAA 12. Our objectives were to further characterize and enable marker-assisted selection (MAS) of the Pse-2 gene in ZAA 12 purported to have broad effect against multiple Psp races. A recombinant inbred population, ZAA 12 × ‘Canadian Wonder’, was challenged by the halo blight pathogen differential set consisting of nine Psp races. The resistance conferred by Pse-2 to races 2, 3, 4, 5, 7, 8, and 9 expanded the effect known for this gene from three to seven races. Segregation in F2 populations confirmed dominant inheritance for Pse-2 against all races except Race 8. Resistance to Race 8 was recessively inherited and most closely fit a 7 resistant to 9 susceptible segregation ratio in the F2 generation. Perhaps other genes are segregating that modify the effect of Pse-2 against Race 8. A sequence characterized amplified region (SCAR) marker tightly linked with Pse-2 was generated. The marker was used to integrate Pse-2 to chromosome 10. A survey of lines and cultivars revealed the SCAR will have broad utility for MAS of Pse-2.

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Copyright © 2011. Copyright © by the Crop Science Society of America, Inc.