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This article in CS

  1. Vol. 52 No. 2, p. 795-806
     
    Received: Nov 5, 2010


    * Corresponding author(s): sandra.andrzejewski@labo-vanilledetahiti.pf
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doi:10.2135/cropsci2010.11.0634

Genetic Linkage Map and Diversity Analysis of Tahitian Vanilla (Vanilla ×tahitensis, Orchidaceae)

  1. Sandra Lepers-Andrzejewski *a,
  2. Sandrine Causse *b,
  3. Bernard Caromelc,
  4. Maurice Wongd and
  5. Michel Drone
  1. a Etablissement Vanille de Tahiti, Département Recherche et Développement, BP 912, 98735 Uturoa, Raiatea, French Polynesia
    b Etablissement Vanille de Tahiti, Département Recherche et Développement, BP 912, 98735 Uturoa, Raiatea, French Polynesia, present address: UMR177 IRD/CIRAD, TA A-17/G, Campus international de Baillarguet, 34398 Montpellier cedex 5, France
    c Institut National de la Recherche Agronomique, UR1052, Unité de Génétique et d'Amélioration des Fruits et Légumes, BP 94, 84143 Montfavet cedex, France
    d Etablissement Vanille de Tahiti, Département Recherche et Développement, BP 912, 98735 Uturoa, Raiatea, French Polynesia, present address: Service du Développement Rural, Département de la recherche agronomique, BP 100, 98713 Papeete, Tahiti, Polynésie française
    e Institut de Biotechnologie des plantes, Bât 630, Université de Paris XI-Orsay, 91405 Orsay cedex, France

Abstract

The first vanilla genetic linkage map was constructed by analyzing the progeny of an interspecific cross between Vanilla ×tahitensis J. W. Moore (pro sp.) and Vanilla pompona Schiede. The two-way pseudotestcross mapping strategy was used and a total of 225 amplified fragment length polymorphism (AFLP) markers were mapped on 18 linkage groups covering 1035.85 cM. Thirteen tahitian vanilla morphotypes, one Vanilla planifolia Andrews and recent hybrids were compared by measuring seven morphological traits and by genotyping, with 539 AFLP fragments. The morphotypes studied showed relatively homogenous stem traits but differed in their bean and leaf traits as in their AFLP pattern. Genetic variability and AFLP genotyping comparison suggest a diversification in French Polynesia based on sexual reproduction and are consistent with the hypothesis of a single introduction event. These results are the first step for vanilla breeding program and will be also useful for tahitian vanilla resource preservation.

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