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Crop Science Abstract - Crop Breeding & Genetics

Mapping Quantitative Trait Loci for Partial Resistance to Powdery Mildew in an Australian Barley Population


This article in CS

  1. Vol. 52 No. 3, p. 1021-1032
    Received: Aug 13, 2011

    * Corresponding author(s): l.hickey@uq.edu.au
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  1. Lee T. Hickey a,
  2. Wendy Lawsonb,
  3. Greg J. Platzb,
  4. Ryan A. Fowlerb,
  5. Vivi Ariefa,
  6. Mark Dietersa,
  7. Silvia Germánc,
  8. Susan Fletcherd,
  9. Robert F. Parke,
  10. Davinder Singhe,
  11. Silvia Pereyrac and
  12. Jerome Franckowiakb
  1. a The University of Queensland, School of Agriculture and Food Sciences, Brisbane, QLD 4072, Australia
    b Department of Employment, Economic Development and Innovation, Hermitage Research Facility, Warwick, QLD 4370, Australia
    c Instituto Nacional de Investigación Agropecuaria, La Estanzuela, Colonia, CP 70000, Uruguay
    d Department of Employment, Economic Development and Innovation, Leslie Research Facility, Toowoomba, QLD 4350, Australia
    e The University of Sydney, Plant Breeding Institute Cobbitty, Narellan, NSW 2570, Australia


Genomic regions influencing resistance to powdery mildew [Blumeria graminis (DC.) E.O. Speer f. sp. hordei Ém. Marchal] were detected in a doubled haploid (DH) barley (Hordeum vulgare L.) population derived from a cross between the breeding line ND24260 and cultivar Flagship when evaluated across four field environments in Australia and Uruguay. Significant quantitative trait loci (QTL) for resistance to B. graminis were detected on six of the seven chromosomes (1H, 2H, 3H, 4H, 5H, and 7H). A QTL with large effect donated by ND24260 mapped to the short arm of chromosome 1H (1HS) conferring near immunity to B. graminis in Australia but was ineffective in Uruguay. Three QTL donated by Flagship contributed partial resistance to B. graminis and were detected in at least two environments. These QTL were mapped to chromosomes 3H, 4H, and 5H (5HS) accounting for up to 18.6, 3.4, and 8.8% phenotypic variation, respectively. The 5HS QTL contributed partial resistance to B. graminis in all field environments in both Australia and Uruguay and aligned with the genomic region of Rph20, a gene conferring adult plant resistance (APR) to leaf rust (Puccinia hordei Otth), which is found in some cultivars having ‘Vada’ or ‘Emir’ in their parentage. Selection for favorable marker haplotypes within the 3H, 4H, and 5H QTL regions can be performed even in the presence of single (major) gene resistance. Pyramiding such QTL may provide an effective and potentially durable form of resistance to B. graminis.

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