A Rapid, Small-Scale Sedimentation Method to Predict Breadmaking Quality of Hard Winter Wheat
- Bradford W. Seabourn *a,
- Zhihua S. Xiaoa,
- Michael Tilleya,
- Thomas J. Heralda and
- Seok-Ho Parkb
- a USDA-ARS, Center for Grain & Animal Health Research, Hard Winter Wheat Quality Laboratory, 1515 College Avenue, Manhattan, KS 66502
b International Delights LLC, 230 Brighton Road, Clifton, NJ 07012. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer
Breeders and processors are always looking for rapid and accurate methods to evaluate wheat (Triticum aestivum L.) quality. The objective of this study was to develop a rapid, small-scale method to accurately determine breadmaking quality for early generation hard winter wheat (HWW) breeding lines by combining the solutions used in the sodium dodecyl sulfate (SDS) sedimentation method (American Association of Cereal Chemists [AACC] 56-70 [AACC, 2000]) and the centrifugation process found within the solvent retention capacity (SRC) method (AACC 56-11 [AACC, 2000]). A preliminary test of the hybrid SDS-SRC method was performed on eight HWW varieties and compared to AACC (56-70) and in-house Hard Winter Wheat Quality Laboratory (HWWQL) methods to show proof of concept. Further validation of the hybrid method was conducted on a diverse set of 53 HWW varieties. The hybrid method was performed in 66% less time than AACC 56-70 and HWWQL methods. Furthermore, sample size was reduced from 6 g for the AACC method to 1 g for the hybrid method. Results obtained from the hybrid method exhibited a higher correlation to bread loaf volume (r ≥ 0.84) compared to results from the AACC method (r > 0.42) and HWWQL method (r ≥ 0.64) for wheat flour. Due to enhanced speed, accuracy, and simplicity the hybrid SDS-SRC sedimentation method may prove useful in breeding programs, grain elevators, and other scenarios where rapid assessment of end-use quality determination is required.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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