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This article in CS

  1. Vol. 52 No. 5, p. 2109-2114
     
    Received: Jan 24, 2012


    * Corresponding author(s): wfehr@iastate.edu
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doi:10.2135/cropsci2012.01.0042

Inheritance and Molecular Mapping of an Allele Providing Resistance to a Cowpea Mild Mottle-Like Virus in Soybean

  1. Ryan C. Bracea,
  2. Walter R. Fehr *a and
  3. Michelle A. Grahamb
  1. a Dep. of Agronomy, Iowa State Univ., Ames, IA 50011-1010. This journal paper of the Iowa Agric. and Home Econ. Exp. Stn., Ames, IA, Project No. 5103 was supported by the Hatch Act, State of Iowa, Iowa Soybean Association, United Soybean Board, Raymond F. Baker Center for Plant Breeding, and Pioneer Hi-Bred International, Inc
    b Corn Insect and Crop Genetics Research Unit and USDA-ARS, Iowa State Univ., Ames, IA 50011-1010. This journal paper of the Iowa Agric. and Home Econ. Exp. Stn., Ames, IA, Project No. 5103 was supported by the Hatch Act, State of Iowa, Iowa Soybean Association, United Soybean Board, Raymond F. Baker Center for Plant Breeding, and Pioneer Hi-Bred International, Inc

Abstract

Damage to soybean [Glycine max (L.) Merr.] from a Cowpea mild mottle-like virus (CPMMLV) (family Betaflexiviridae and genus Carlavirus) has been of increasing concern in Argentina, Brazil, Mexico, and Puerto Rico. Soybean cultivars and lines differing in their reaction to the virus have been identified. The first objective of our study was to determine the genetic basis of resistance and susceptibility of F1 plants and 100 F2–derived lines from the cross of ‘IA3023’ (resistant) × ‘IA3024’ (susceptible) using natural field infection in Puerto Rico and Mexico during 2011. All of the F1 plants were susceptible, and the segregation of F2–derived lines satisfactorily fit a ratio of 3 susceptible:1 resistant, which indicated that resistance was controlled by a recessive allele at a single locus. The second objective of our study was to determine the genetic location of the locus by genotyping 93 of the F2–derived lines. The molecular analysis identified a locus on chromosome 18 (linkage group G) at the same estimated genetic position as the marker BARCSOYSSR_18_0443 (89.8 cM). The locus was flanked by BARCSOYSSR_18_0456 (88.6 cM) and BARCSOYSSR_18_0458 (91.0 cM). The locus was designated Rbc1 and the resistance allele in IA3023 was identified as rbc1. Markers tightly linked to the Rbc1 locus can be used to select resistant progeny in segregating populations from crosses between resistant and susceptible parents.

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