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This article in CS

  1. Vol. 52 No. 5, p. 2121-2130
     
    Received: Jan 30, 2012


    * Corresponding author(s): agrcpp@ku.ac.th
    chalermpol.ph@gmail.com
    agrcsr@yahoo.com
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doi:10.2135/cropsci2012.01.0058

Molecular Characterization of a Spontaneous Waxy Starch Mutation in Cassava

  1. Pornsak Aiemnakaab,
  2. Arunee Wongkaewa,
  3. Jumnong Chanthaworna,
  4. Sathakupt Ken Nagashimaa,
  5. Supawadee Boonmaa,
  6. Jetsada Authapuna,
  7. Sujin Jenweerawata,
  8. Pasajee Kongsilaa,
  9. Piya Kittipadakula,
  10. Sutkhet Nakasathiena,
  11. Tanee Sreewongchaia,
  12. Wannasiri Wannaratc,
  13. Vichan Vichukitab,
  14. Luís Augusto Becerra López-Lavalled,
  15. Hernán Ceballosd,
  16. Chareinsuk Rojanaridpiched *ab and
  17. Chalermpol Phumichai *ab
  1. a Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
    b Center for Advanced Studies in Agriculture and Food, KU Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand
    c Plant Tissue Laboratory, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, Bangkok 10900, Thailand
    d International Center of Tropical Agriculture (CIAT), Recta Cali-Palmira Km 17, Cali, Colombia

Abstract

Cassava (Manihot esculenta Crantz) starch is traded in international markets more than starch from any other source. The starch industry requires cassava cultivars with novel starch characteristics for commercial exploitation. A natural source of waxy (amylose-free) cassava starch, clone AM 206-5, was identified at the International Center for Tropical Agriculture (CIAT). The granule-bound starch synthase I (GBSSI) gene is related to the waxy starch trait. The objectives of this study were to introgress the waxy starch mutation into Thailand's cassava breeding populations, to analyze the inheritance of waxy starch in cassava, and to develop molecular markers for this trait. AM 206-5 (wxwx) was crossed with several elite cultivars (WxWx) to transfer the wx allele. Unrelated “F1” (Wxwx) genotypes were crossed among themselves to produce a “F2” segregating population with negligible levels of inbreeding depression. The waxy starch phenotype was recovered in 25.7% of the 11,192 genotypes tested. This result provides further evidence that cassava is a functional diploid and that only one copy of GBSSI is present in cassava. A full-length genomic DNA sequence of GBSSI was isolated and characterized from C8, a waxy starch “F2” clone derived from AM 206-5. We exploited these sequence differences to develop two diagnostic single-nucleotide-amplified polymorphism (SNAP) markers to differentiate homozygous waxy (wxwx) from the heterozygous (Wxwx) and homozygous (WxWx) nonwaxy genotypes.

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Copyright © 2012. Copyright © by the Crop Science Society of America, Inc.