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This article in CS

  1. Vol. 8 No. 3, p. 269-272
    Received: Oct 13, 1967

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Self-Compatibility Studies with Diploid Alsike Clover, Trifolium hybridum L. III. Response to Temperature1

  1. C. E. Townsend2



The compatibility reactions of two diploid St alsike clover clones, 6–5 from the Danish variety ‘Otofte’ and 7–1 from an Ohio-grown seed lot, were temperature-sensitive in controlled environmental chambers. One to 2 days at constant 32 C changed the compatibility reaction of genotype 6–5 from self-incompatibility to self-compatibility. However, 2 to 3 days at 32 day-27 C night temperatures were required to change the compatibility reaction of genotype 7–1. By the 4th day of treatment, 35 and 62% of the selfed florets were setting seed at the constant 32 C treatment for genotypes 6–5 and 7–1, respectively. Seed set for genotype 6–5 reached a high of 20% on the 5th day at the 32 day-27 C night treatment; 11% of the florets set seed on the 4th day at the 32 day-21 C night treatment; and a 4% seed set was reached on the 3rd day at the constant 27 C treatment. Genotype 7–1 did not respond to the 32 day-21 C night and constant 27 C temperature treatments. After the compatibility reaction was changed at the high temperatures, propagules of the two genotypes were removed and placed at 21 C. The compatibility reaction of genotype 6–5 changed immediately to self.lncompatibility. However, 24 hr were required to change the compatibility reaction of genotype 7–1 from one of self-compatibility to one of self-incompatibility. The site of the change in the compatibility reaction for genotype 7–1 was the style, but it was not possible to locate the site for genotype 6–5.

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