Limitations of Glutamic Acid Decarboxylase Activity for Estimating Viability in Beans (Phaseolus Vulgaris L.)1
- Edwin James1
Glutamica cid decarboxylase activity (GADAi)n whole beans stored at 32.2 C and 85 to 90% relative humidity, and on the axes and cotyledons of viable and deteriorated seeds of five varieties of beans, was determined by a manometric method for measuring the evolution of CO2. GADA in some varieties remained at a high level in seeds which had lost viability. Correlation between GADA and germinations yielded coefficients ranging from +0.39 N.S. for ‘Tendercrop’ to +0.89** for ‘Taylor’. Determinations of GADA on the cotyledons and axes showed that the cotyledons contributed 87% of the activity in viable seeds and 91% in deteriorated seeds. The low correlation coefficients and the continuing high activity in the cotyledons limit the use of GADA in determining quality in the varieties used.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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