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This article in CS

  1. Vol. 8 No. 4, p. 403-404
     
    Received: Oct 25, 1967


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doi:10.2135/cropsci1968.0011183X000800040001x

Limitations of Glutamic Acid Decarboxylase Activity for Estimating Viability in Beans (Phaseolus Vulgaris L.)1

  1. Edwin James1

Abstract

Abstract

Glutamica cid decarboxylase activity (GADAi)n whole beans stored at 32.2 C and 85 to 90% relative humidity, and on the axes and cotyledons of viable and deteriorated seeds of five varieties of beans, was determined by a manometric method for measuring the evolution of CO2. GADA in some varieties remained at a high level in seeds which had lost viability. Correlation between GADA and germinations yielded coefficients ranging from +0.39 N.S. for ‘Tendercrop’ to +0.89** for ‘Taylor’. Determinations of GADA on the cotyledons and axes showed that the cotyledons contributed 87% of the activity in viable seeds and 91% in deteriorated seeds. The low correlation coefficients and the continuing high activity in the cotyledons limit the use of GADA in determining quality in the varieties used.

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