Use of Cytoplasmic Male Sterility, Compared to Hand Emasculation, and Translocations for Chromosome Analysis in Sorghum vulgare Pers1
- G. A. Papathanasiou and
- K. J. Lessman2
Results obtained after use of a cytoplasmic male-sterile restorer gene system in testcrosses with translocations for chromosome analysis were compared with those from testcrosses after hand emasculation. The use of male-sterile cytoplasm was relatively easy and less time consuming, thus allowing production of large numbers of progeny and an accurate estimate of linkage intensity.
This variation in methodology may have application with other self-pollinated crops where cytoplasmic male-sterile restorer gene systems and transloeation stocks exist.
Using this scheme the linkage groups T231(13.06±l.1)- Dd and T396(5.0±0.6)-Yy were found, and the possible location of these markers was discussed. The establishment of the chromosome complement and male sterility of MSCK60 as a standard for chromosome analyses in sorghum was proposed.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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