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This article in CS

  1. Vol. 9 No. 5, p. 615-618
    Received: Feb 15, 1969

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A Laboratory Method of Determining Cold Hardiness in Bermudagrass, Cynodon dactylon (L.) Pers.1

  1. R. M. Ahring and
  2. R. M. Irving2



Differences in cold resistance among bermudagrass varieties can be detected by the triphenyl tetrazolium chloride (TTC) test. The TTC test has a limited potential as a tool in screening and predicting survival in areas where certain varieties are not recommended. The freezing technique which supplemented the TTC test showed that: 1) temperatures between –2.2 and –4.4 C caused significant injury to rhizomes of ‘Coastal;’ 2) –6.6 C caused significant injury to common, ‘Midland,’ and ‘Greenfield;’ 3) introductions A-9957 (Yugoslavia) and A-8153 (Afghanistan) were not injured at –6.6 C and occasionally will survive with some freeze injury at –9.4 C; 4) a 25% reduction in TTC absorbance following freeze treatment correlated closely with visual survival checks and served as a good index of viability; 5) cold hardiness may be induced in certain strains of bermudagrass in response to short-day lengths and further conditioned by low temperatures under field conditions; and 6) rhizomes acclimated to fall and winter field conditions are more cold resistant than those grown under short day lengths and warm temperatures.

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