Genetic Control of Phytoglycogen Accumulation in Maize (Zea mays L.)1
- John E. Ayers and
- Roy G. Creech2
Investigations of the role of the genes ae and su2 in phytoglycogen accumulation in maize endosperms were conducted by quantitating and characterizing the watersoluble polysaccharides (WSP) from the 16 genotypes of endosperm resulting from all possible crosses betweenlines homozygoufso r normal(Ae Su), ae, su1, and ae su1. The WSP was extracted in a mercuric chloride solution and enzymatically hydrolyzed to glucose, then measured as reducing sugar.
Only the endosperms homozygous for su1 contained phytoglycogen. Increased doses of ae decreased the amount of phytoglycogen at 20 days after pollination from 38.8 to 4.2% of the dry weight. The β-amylolysis limit of the phytoglycogen from the double mutant suggested that it may be a more loosely branched polysaccharide.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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