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Rhizobia strains, original host, site of origin, proposed taxonomic position, and plant growth promoting activity.

Strain identification Original host Source of strains Proposed taxonomic position Phosphate solubilization‡ Indole acetic acid production†
102A13 Medicago arabica Costa Rica Sinorhizobium medicae +
102B11 Medicago polymorpha Florida, U.S. S. medicae + +
102H2 Medicago rugosa Desr. Tunisia S. medicae +
WSM540 M. polymorpha Italy S. medicae +
W118 M. rugosa Australia S. medicae +
WSM1115§ M. polymorpha Greece S. medicae + +
M2 Medicago lupulina United States S. medicae +
M18 Medicago rigidula Syria Sinorhizobium meliloti
102F85§ Medicago sativa Canada S. meliloti + +
M49§ M. rigidula Syria S. meliloti
RR1128 Medicago truncatula Australia S. meliloti
The strains 102A13, 102B11, 102H2, WSM540, and W118 are blended together in the Nitragin N inocula (EMD Crop Bioscience, Novozymes, Brookfield, WI). Strain 102F85 is commercially available as Nitragin A. Strains WSM1115, M2, and M49 are available by Plant Probiotics (Indianapolis, IN). M18 is an experimental strain and RR1128 is a commercial strain for alfalfa received from Australia by R. Ballard in 2010. Basic information about the strains was obtained by J. Kosamke, EMD Crop Bioscience, R. Ballard, South Australian Research and Development Institute (Adelaide, SA), and T. Wacek, Plant Probiotics (Indianapolis, IN) (personal communication, 2010).
+ indicates positive for phosphate solubilization or indole acetic acid production; – indicates no phosphate solubilization or indole acetic acid production.
§Strains in bold were used for the NFmix3 blend (WSM1115, 102F85, and M49) to test the interaction of blending strains.