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Journal of Animal Science Abstract - Ruminant Nutrition

1607 Effect of nitrate, monensin, and the combination of additives on rumen fermentation using a semicontinuous culture system.


This article in JAS

  1. Vol. 94 No. supplement5, p. 781-782
    Published: November 9, 2016


  1. M. Capelari *1,
  2. K. A. Johnson2,
  3. B. Latack1,
  4. J. Roth1 and
  5. W. Powers1
  1. 1 Michigan State University, East Lansing
    2 Washington State University, Pullman


A 37-d experiment was conducted to investigate the effect of nitrate (NIT) and monensin (MON) on rumen fermentation using a semicontinuous culture system. We hypothesized that the combination of additives would reduce CH4 emissions beyond that of either additive alone, without affecting production parameters. Additives (0, 1.25, and 2.5% diet DM of NIT; 0 and 4 mg/L of MON) were tested alone and in combination (NIT+MON; 6 total treatments; 3 replicates/treatment). Buffer and water were added to eighteen 2.2-L vessels on d −8 (1.2 L of a 50:50 mixture). The first 7 d (d −7 to 0) served as a steady state phase. On d −7, rumen fluid was pooled from 5 nonadapted lactating cows fed a 50:50 forage to concentrate diet and filtered through 2 layers of cheesecloth, and 1 L was transferred to each vessel along with 30 g of solid rumen content and 20 g of a basal diet (50:50 forage to concentrate) in a 8 by 20 cm nylon bag (50 μm mesh size). On d −6, 20 g of the basal diet replaced the solid rumen content bag, and from this point onward, 2 bags, each containing 20 g of the treatment diet, were always present in the vessels for 48 h. Buffer was infused at a constant rate (70 mL/h) throughout the experiment with a peristaltic pump. Gas production was measured daily. Twice weekly, DM disappearance (DMD), pH, and ammonia nitrogen (NH3–N) were measured. Treatment did not affect DMD (69.13 g/100 g DM; P > 0.05). Compared with the negative control treatment, addition of NIT reduced total gas production (2.84 vs. 3.03 L/d; P = 0.02), CH4 production (28.65 vs. 40.9 mM/d; P < 0.01), and CO2 production (87.57 vs. 97.36 mM/d; P < 0.05). Compared with the negative control, addition of MON reduced CH4 production (29.4; P < 0.01). Further CH4 reduction, compared with the negative control, was observed when NIT+MON was added (24.31 vs. 40.9 mM; P = 0.04). No treatment effects were observed for pH (7.1) or protozoa count (4.3 × 103). Addition of NIT reduced NH3–N (16.65 vs. 23.5 mg/dL; P < 0.01). The combination of NIT+MON enhanced reduction of CH4 production in a semicontinuous culture fed a 50:50 forage:concentrate diet, with no detriment to DMD.

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