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This article in JEQ

  1. Vol. 22 No. 3, p. 620-624
     
    Received: Sept 5, 1991


    * Corresponding author(s):
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doi:10.2134/jeq1993.00472425002200030029x

Sewage Sludge Proteins: I. Extraction Methodology

  1. R. N. Lerch *,
  2. K. A. Barbarick,
  3. P. Azari,
  4. L. E. Sommers and
  5. D. G. Westfall
  1. C ropping Systems and Water Quality Research Unit, USDA-ARS, Columbia, MO 65211;
    D ep. of Agronomy, Colorado State Univ., Ft. Collins, CO 80523.
    D ep. of Biochemistry, Colorado State Univ., Ft. Collins, CO 80523.

Abstract

Abstract

The extraction and quantitation of sewage sludge proteins is a pre-requisite to evaluating their role as labile C and N sources in sludge-amended soil. The objective of this study was to develop extraction methodology for the routine quantitation of sewage sludge proteins. Seven sewage sludges were obtained and prepared by oven drying at 55 °C followed by extensive grinding and mixing to produce homogenous samples. Proteins were extracted using H2O, 10% (v/v) Triton X-100 (a non-ionic detergent), and 1 M NaOH, and they were analyzed using the Lowry protein assay with bovine serum albumin standards. Protein contents of extracts ranged from (in g kg−1, dry wt. basis): (i) H2O, 1.3 to 24; (ii) 10% Triton X-100, 3.6 to 59; and (iii) 1 M NaOH, 53 to 280. Water and detergent-extractable proteins were less than, and base-extractable proteins were within, literature values reported for total sludge proteins. The coefficients of variation (CV) for each extractant and all sludges were, with one exception, below 6%. The three methodologies provided precise and reproducible data for the extraction of sewage sludge proteins. The detergent extraction procedure is recommended for routine use because of its shorter extraction time.

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