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Journal of Environmental Quality Abstract - Waste Management

Enzymatic Characterization of Organic Phosphorus in Animal Manure


This article in JEQ

  1. Vol. 30 No. 5, p. 1685-1692
    Received: Dec 8, 2000

    * Corresponding author(s): zhe@maine.edu
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  1. Zhongqi He * and
  2. C. Wayne Honeycutt
  1. USDA Agricultural Research Service, New England Plant, Soil, and Water Lab., Univ. of Maine, Orono, ME 04469


Information on the forms of P present in animal manure may improve our ability to manage manure P. In most investigations of manure P composition, only inorganic and total P are determined, and the difference between them is assigned as organic P. In this study, we explored the possibility of identifying and quantifying more specific organic P forms in animal manure with orthophosphate-releasing enzymes. Pig (Sus scrofa) manure and cattle (Bos taurus) manure were first sequentially fractionated into water-soluble P, NaHCO3–soluble P, NaOH-soluble P, HCl-soluble P, and residual P. The fractions were separately incubated with wheat phytase, alkaline phosphatase, nuclease P1, nucleotide pyrophosphatase, or their combinations. The released orthophosphate was determined by a molybdate blue method. Part of the organic P in those fractions could be identified by the enzymatic treatments as phytate (i.e., 39% for pig manure and 17% for cattle manure in water-soluble organic P), simple phosphomonoesters (i.e., 43% for pig manure and 15% for cattle manure in NaOH-soluble organic P), nucleotide-like phosphodiesters (2–12%), and nucleotide pyrophosphate (0–4%). Our data indicate that the enzymatic treatment is an effective approach to identify and quantify the organic P forms present in animal manures.

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Copyright © 2001. American Society of Agronomy, Crop Science Society of America, Soil Science SocietyPublished in J. Environ. Qual.30:1685–1692.