Evaluation of Phosphorus Characterization in Broiler Ileal Digesta, Manure, and Litter Samples: 31P-NMR vs. HPLC
- A. B. Leytem *a,
- P. Kwanyuenb,
- P. W. Plumsteadc,
- R. O. Maguired and
- J. Brakec
- a USDA-ARS, Northwest Irrigation and Soils Research Lab., 3793 N. 3600 E., Kimberly, ID 83341-5076
b USDA-ARS, Soybean and Nitrogen Fixation Research Unit, 3127 Ligon St., Raleigh, NC 27607
c Dep. of Poultry Science, College of Agriculture and Life Sciences, North Carolina State Univ., Raleigh, NC 27695-7608
d Crop and Soil Environmental Sciences Dep., Virginia Tech, Blacksburg, VA 24061
Using 31-phosphorus nuclear magnetic resonance spectroscopy (31P-NMR) to characterize phosphorus (P) in animal manures and litter has become a popular technique in the area of nutrient management. To date, there has been no published work evaluating P quantification in manure/litter samples with 31P-NMR compared to other accepted methods such as high performance liquid chromatography (HPLC). To evaluate the use of 31P-NMR to quantify myo-inositol hexakisphosphate (phytate) in ileal digesta, manure, and litter from broilers, we compared results obtained from both 31P-NMR and a more traditional HPLC method. The quantification of phytate in all samples was very consistent between the two methods, with linear regressions having slopes ranging from 0.94 to 1.07 and r 2 values of 0.84 to 0.98. We compared the concentration of total monoester P determined with 31P-NMR with the total inositol P content determined with HPLC and found a strong linear relationship between the two measurements having slopes ranging from 0.91 to 1.08 and r 2 values of 0.73 to 0.95. This suggests that 31P-NMR is a very reliable method for quantifying P compounds in manure/litter samples.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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