Quantifying Nitrogen Process Rates in a Constructed Wetland Using Natural Abundance Stable Isotope Signatures and Stable Isotope Amendment Experiments
- Dirk V. Erler *a and
- Bradley D. Eyrea
This study describes the spatial variability in nitrogen (N) transformation within a constructed wetland (CW) treating domestic effluent. Nitrogen cycling within the CW was driven by settlement and mineralization of particulate organic nitrogen and uptake of NO3 − The concentration of NO3 − was found to decrease, as the δ15N-NO3 − signature increased, as water flowed through the CW, allowing denitrification rates to be estimated on the basis of the degree of fractionation of δ15N-NO3 − Estimates of denitrification hinged on the determination of a net isotope effect (η), which was influenced by processes that enrich or deplete 15NO3 − (e.g., nitrification), as well as the rate constants associated with the different processes involved in denitrification (i.e., diffusion and enzyme activity). The influence of nitrification on η was quantified; however, it remained unclear how η varied due to variability in denitrification rate constants. A series of stable isotope amendment experiments was used to further constrain the value of η and calculate rates of denitrification, and nitrification, within the wetland. The maximum calculated rate of denitrification was 956 ± 187 μmol N m−2 h−1, and the maximum rate of nitrification was 182 ± 28.9 μmol N m−2 h−1 Uptake of NO3 − was quantitatively more important than denitrification throughout the wetland. Rates of N cycling varied spatially within the wetland, with denitrification dominating in the downstream deoxygenated region of the wetland. Studies that use fractionation of N to derive rate estimates must exercise caution when interpreting the net isotope effect. We suggest a sampling procedure for future natural abundance studies that may help improve the accuracy of N cycling rate estimates.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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