About Us | Help Videos | Contact Us | Subscriptions
 

Journal of Environmental Quality Abstract - Waste Management

Levels of Antibiotic Resistance Genes in Manure, Biosolids, and Fertilized Soil

 

This article in JEQ

  1. Vol. 40 No. 1, p. 248-255
     
    Received: May 7, 2010


    * Corresponding author(s): xagorara@msu.edu
 View
 Download
 Alerts
 Permissions
Request Permissions
 Share

doi:10.2134/jeq2010.0209
  1. Mariya Munir and
  2. Irene Xagoraraki *
  1. Dep. of Civil and Environmental Engineering, Michigan State Univ., East Lansing, MI 48824. Assigned to Associate Editor Lakhwinder Hundal.

Abstract

Increasing antibiotic resistance genes in the environment may pose a threat to public health. In this study, tetracycline and sulfonamide resistance genes (Tet-W, Tet-O, and Sul-I) were quantified in 24 manure samples from three farms and 18 biosolids samples from seven different wastewater treatment plants using quantitative polymerase chain reaction methods. Concentrations of Tet-W and Tet-O genes were observed to be significantly higher (p < 0.05) in manure than in biosolids samples. The background soil samples showed significantly lower concentration of the above genes compared with manure and biosolids. Lime-stabilized biosolids showed significantly (p < 0.05) lower concentration of antibiotic resistance genes compared with other biosolids treatment methods. Elevated levels of antibiotic resistance genes (Tet-W, Tet-O, and Sul-I) were observed in the amended soil samples after the land application of manure or biosolids (Site A) monitored for a period of about 4 mo. However, at another site (Site B), no significant increase (p > 0.05) in concentration of antibiotic resistance genes was observed after biosolids application on soil. Even though the concentration of antibiotic resistance genes in manure was statistically higher than that in biosolids, when they were applied on land, the contribution to the soil depended on the background soil concentration and the soil characteristics. Further study of multiple soil samples in various locations is needed.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © 2011. American Society of Agronomy, Crop Science Society of America, Soil Science SocietyAmerican Society of Agronomy, Crop Science Society of America, and Soil Science Society of America