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This article in JEQ

  1. Vol. 42 No. 2, p. 464-473
     
    Received: Aug 21, 2012
    Published: February 11, 2013


    * Corresponding author(s): gerwin.koopmans@wur.nl
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doi:10.2134/jeq2012.0322

Characterization of Colloidal Phosphorus Species in Drainage Waters from a Clay Soil Using Asymmetric Flow Field-Flow Fractionation

  1. Inge C. Regelinka,
  2. Gerwin F. Koopmans *ab,
  3. Caroline van der Salmb,
  4. Liping Wenga and
  5. Willem H. van Riemsdijka
  1. a Dep. of Soil Quality, Wageningen Univ., P.O. Box 47, 6700 AA, Wageningen, the Netherlands
    b Alterra, Wageningen Univ. and Research Centre, P.O. Box 47, 6700 AA, Wageningen, the Netherlands

Abstract

Phosphorus transport from agricultural land contributes to eutrophication of surface waters. Pipe drain and trench waters from a grassland field on a heavy clay soil in the Netherlands were sampled before and after manure application. Phosphorus speciation was analyzed by physicochemical P fractionation, and the colloidal P fraction in the dissolved fraction (<0.45 μm) was analyzed by asymmetric flow field-flow fractionation (AF4) coupled to high-resolution inductively coupled plasma–mass spectrometry and ultraviolet diode array detector. When no manure was applied for almost 7 mo, total P (TP) concentrations were low (<21 μmol L−1), and TP was almost evenly distributed among dissolved reactive P (DRP), dissolved unreactive P (DUP), and particulate P (PP). Total P concentrations increased by a factor of 60 and 4 when rainfall followed shortly after application of cattle slurry or its solid fraction, respectively. Under these conditions, DRP contributed 50% or more to TP. The P speciation within the DUP and PP fractions varied among the different sampling times. Phosphorus associated with dissolved organic matter, probably via cation bridging, comprised a small fraction of DUP at all sampling times. Colloidal P coeluted with clay particles when P application was withheld for almost 7 mo and after application of the solid cattle slurry fraction. At these sampling times, PP correlated well with particulate Fe, Al, and Si, indicating that P is associated with colloidal clay particles. After cattle slurry application, part of DUP was probably present as phospholipids. Physicochemical fractionation combined with AF4 analysis is a promising tool to unravel the speciation of colloidal P in environmental water samples.

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Copyright © 2013. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.